WebMay 12, 2024 · Automated chemical synthesis of oligonucleotides is of fundamental importance for the production of primers for the polymerase chain reaction (PCR), for … WebApr 12, 2024 · The surface density 3.9 fmol/mm 2 of the GPIHBP1 bound LPL was sufficient to observe the interaction while a measurable association was detected when the surface density of neutravidin-bound biotin-bLPL was 19.5 fmol/mm 2. When measuring 50 mg/ml BSA binding, the steady-state bound BSA/LPL molar ratio was 0.12:1 for neutravidin …
Measurement unit conversion: femtomole - Convert Units
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WebUsing the stable isotope-labeled (SI) peptide as an internal control, absolute concentrations of plasma APL1β peptide could be quantified as several hundred amol/mL. To our knowledge, this is the lowest detection level of endogenous plasma peptide quantified by SRM/MRM. KEYWORDS: SRM/MRM low abundant plasma peptide peptide enrichment WebFeb 22, 2024 · Protein phosphorylation plays vital roles in the regulation of various biological processes involving in protein folding, molecular recognition, cell growth, and metabolism. It is prerequisite to develop effective enrichment methods of trace phosphopeptides before mass spectrometry (MS) analysis. In … WebMar 27, 2024 · Detection of mRNA m 6 A by m 6 A-Rol-LAMP. We initially used two pairs of RNA oligos with or without m 6 A modifications, Oligo-1-A/Oligo-1-m 6 A and Oligo-2-A/Oligo-2-m 6 A, to test the feasibility of our designed method. Bst 2.0 DNA polymerase and T3 DNA ligase were initially used for the elongation and sealing step of padlock … eagle claw baitholder hooks