Polyhedrin promoter sequence
Web[目的]通过选用哺乳动物细胞特异性启动子—人类延伸因子1α启动子(Elonggation factor 1α promoter,EF1-α)、利用病毒假型化工具—截断型水疱型口膜炎病毒G蛋白(Truncated Vessicular stomatitis virus protein G,VSV-GED)、添加转录后调 WebApr 10, 2024 · PH promoter: AcMNPV polyhedrin promoter. It drives high-level expression of the gene encoding your recombinant protein. ORF: The open reading frame of your gene …
Polyhedrin promoter sequence
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WebModified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat α2,6-sialyltransferase (ST6Gal I) in silkworm. Modified … WebA guide to Addgene's empty vector backbones curated by species, epitope tags/fusion protein, choosing marker, viral expression, and more.
WebNov 1, 2001 · A series of deletions in the upstream region of the gene encoding polyhedrin (polh) of Bombyx mori nucleopolyhedrovirus (BmNPV) were generated in plasmid … WebDec 23, 1987 · A sequence 69 nucleotides upstream to the normal position of the polyhedrin ATG translation initiation codon was sufficient for maximum promoter activity but this …
Web6xHis tag. The polyhedrin promoter controls expression of your recombinant protein in insect cells. Map The figure below summarizes the features of the vector. Details of the multiple cloning site are shown on page 3. The complete sequence can be downloaded from our web site (www.invitrogen.com) or requested from Technical Service (page 10 WebBaculovirus transfer plasmid with the polyhedrin promoter, encoding an optional C-terminal 6xHis tag. Baculovirus transfer plasmid with the polyhedrin promoter, encoding ...
WebThe pLSG-IBA168 carrier is designed for high-level phrase of target murine with a C-terminal Twin-Strep-tag® and FLAG-tag in insect cells.The…
Webin insect cells is investigated in this study. Expression in baculovirus-infected cells of a sequence encoding the C and E1 structural proteins under the control of the very late promoter P10 (AcSLP10-C-E1) led to the synthesis of … dyi metal ceiling fan light shadesWebThese elements included CAG promoter, MHC Class I signal sequence (MHCIss), MHC Class I trafficking domain (MITD), and Woodchuck hepatitis virus posttranscriptional regulatory … dyi mixed wire copper bracelet videoWebBaculoviruses having proven to be the most powerful and versatile eukaryotic expression vectors available. All unique laboratory manual is designed to search both beginning and experienced researchers construct and use baculovirus vector scheme. I simplifies selection of the most appropriate baculovirus vector scheme for an given problem, then … crystal radio companyWebWe noticed that marine mussel has silk-like domains containing many repeats with abundance of glycine and alanine. In the present work, we redesigned mussel-derived silk-like gene sequence which contains alternating repeated and nonrepeated regions with optimized codons for Escherichia coli. For successful expression of recombinant mussel … crystal radio constructionWebMar 31, 2015 · Since the final aim was to produce recombinant AgMNPVs orally infectious (polh +) to A. gemmatalis larvae, pI3 was designed to contain the polh ORF (preceded by p10 5'-untranslated sequence) under the control of the p10 promoter and followed by the polh downstream region, including the transcriptional termination signal. dyi motorized ornament hangersWebMay 13, 2014 · The expression cassette consisted of a cDNA encoding for the baculovirus transactivation factors IE1 and IE0, expressed under the control of the polyhedrin … dyi microwave pot holdersWebSEQUENCE LISTING. The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Jun. 22, ... Rous … crystal radio doesn\u0027t work